That same week, on May 21, 2021, Dugg released the 19-track mixtape, titled Free Dem Boyz, a dedication to all of his incarcerated friends. In May 2021, Billboard named 42 Dugg the "R&B/Hip-Hop Rookie of the Month". OMB Peezy was later charged with the shooting with aggravated assault with a deadly weapon and possession of a firearm during the commission of a crime. On February 21, 2021, a shooting occurred at Roddy Ricch and 42 Dugg's music video shoot, which left three people injured in Atlanta. Hayes followed this success by doing guest features on songs for other artists such as Marshmello, Lil Keed, Meek Mill, Blac Youngsta, Big Sean, T.I., Kaash Paige, Tory Lanez and Latto. This boosted sales of his mixtape Young & Turnt 2, peaking at number 58 on the Billboard 200 and number six on Billboard 's Independent Albums chart. He was also featured on Lil Baby's track " We Paid" in May 2020, peaking at number 10 on the Billboard Hot 100. 42 Dugg received national attention when he was featured on Lil Baby's February 2020 track " Grace", peaking at number 48 on the Billboard Hot 100. After writing slam poetry with Lil Baby, he signed to Lil Baby's 4 Pockets Full (4PF) and Yo Gotti's Collective Music Group (CMG), in conjunction with Interscope Records. Hayes first gained traction for tracks "The Streets" and "STFU". He grew up listening to Yo Gotti and Jeezy. The numbers "42" in Dugg's name is a numerical reference to a local Crips LC set known as the Hustle Boys. He attended Denby High School in Detroit, Michigan, but later dropped out. Early life ĭion Hayes was born on November 25, 1994, and raised in the Denby neighborhood on Whittier Ave & Wayburn St on the east side of Detroit, Michigan. His fourth mixtape, Free Dem Boyz, was released in 2021, peaking at number 8 on the Billboard 200. His second mixtape Young & Turnt 2 (2020) garnered acclaim and marked his debut on the Billboard 200. He is known for his collaborations with Lil Baby, including " Grace" and " We Paid", the latter becoming his first top 10 on the Billboard Hot 100. He is jointly signed to rappers Lil Baby and Yo Gotti's respective labels 4 Pockets Full (4PF) and Collective Music Group (CMG), in conjunction with Interscope Records. Dion Marquise Hayes (born November 25, 1994), known professionally as 42 Dugg, is an American rapper from Detroit, Michigan.
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Todd Hatlem (Prisoner/Knight’s Attendant) has been involved in the theater since 1974, working on-stage and behind the scenes in set construction, lighting, sound, props, and directing. There is no way she could continue to live her dreams without them. Other roles include Katherine in Freaky Friday, NRACT Jenny in It Shoulda Been You Theatre in the Park, and Angelina in Trial By Jury The Durham Savoyards, as well as the 2nd runner up in Raleigh Little Theater's 2019's Divas! Elizabeth wants to thank her family and friends for their undying love and support. Elizabeth has been performing for over 20 years, studying vocal performance at The Juilliard School and Indiana University: Jacob's School of Music. Her most recent show was Side by Side by Sondheim at NRACT, which was her first show after COVID had shut down the theater after only three performances of her last show in 2020. They’re very excited to be back on stage and honored to work with this cast and crew throughout this experience.Įlizabeth Hankla (Aldonza/Dulcinea) is ecstatic to be making her Justice Theater Project debut. MK Gettinger (Governor’s Aide/Fight Captain) has been working in stage combat and intimacy work since 2019 and is making their first appearance in the tri-state area theater scene with Man of La Macha. He wishes to thank everyone who’s made this production possible and everyone reading this program for coming to see the show! Pinky ( Hairspray), Pirate Ancestor ( The Addams Family), Duncan Durbin ( Aprés Opèra), Number One ( The Girl Who Was Asked to Turn Blue), and Eddy ( The Yellow Boat). Sean Flynn (Jose/Knight’s Attendant) is thrilled to be in his first show with The Justice Theater Project! Sean has been acting in plays for nine years. Thank you all for coming, and enjoy the show! Tim has also appeared onstage with Raleigh Little Theatre, Forest Moon Theatre, and North Raleigh Arts and Creative Theatre. Being part of such a talented cast is always a delight, and he is looking forward to many more productions with all of those involved. Tim Fitch (Pedro) is delighted to make his Justice Theater Project debut with Man of La Mancha. She hopes you enjoy the show just as much as she has enjoyed working on it! Shelby is a proud BFA Musical Theatre graduate of Millikin University and a proud member of the Actor’s Equity membership candidate program. Some of her favorite theater credits include Violet in 9 to 5, Maurice in Madagascar: A Musical Adventure, Laurey in Oklahoma, Millie in Thoroughly Modern Millie, and many more. Shelby Barros (Antonia/Fermina/Horse) is very excited to perform in her first Justice Theater Project show! She has choreographed for the JTP summer camps and house-managed for JTP for many years. In his free time, he writes plays for young audiences! He currently serves as a member of Pure Life Theatre’s Core Leadership and as Seed Art Share’s Managing Director. In 2020 he was recognized as an “Outstanding Arts Educator” by Chatham Life + Style in their publication’s Best of the Year event. As a teaching artist, he has worked with SAY, NCT, CYT, Raleigh Little Theatre, and Applause! Youth Theatre, TAP, The ArtsCenter, Seed Art Share, Burning Coal Theatre, and Starbright Little Players. He’s acted with Seed Art Share, Burning Coal Theatre, Pittsboro Youth Theatre, Theatre in the Park, Pure Life Theatre, and Summer Storybook Theatre. He holds a BFA in Theatre for Youth from East Carolina University. Meet the Cast and Artistic Production Team:ĭylan Bailey (Padre) is a Raleigh-based theater artist and arts educator. His noble cause begs us to question: Is it he or the world that has gone mad, as he dares to Dream the Impossible Dream? His wild antics change the lives of Sancho Panza, his loyal sidekick, and of Aldonza, a prostitute who he sees as the vision of purity, Dulcinea. MAN OF LA MANCHA celebrates the perseverance of a dying old man who imagines himself a gallant knight on a noble quest to right all wrongs. Powerful, brutal, hilarious, and heartbreaking, MAN OF LA MANCHA tells the story of the “mad” knight Don Quixote as a play-within-a-play, performed by Cervantes and his fellow prisoners as he awaits a hearing with the Spanish Inquisition. The original production won the Tony Award for Best Musical. Inspired by Miguel de Cervantes’s 17-century masterpiece novel Don Quixote, MAN OF LA MANCHA is one of the most successful musicals in Broadway history. Written By Dale Wasserman, Music By Mitch Leigh, Lyrics By Joe Darionĭirected by Adam Twiss, Music Direction by Katherine Anderson June 10-26, 2022 Ĩ208 Brownleigh Drive, Raleigh 27617, Umstead Park United Church of Christ Ultimately, this produces an image of your blot with ‘white’ glowing bands on a black background. The chemiluminescent substrate mixture for HRP consists of peroxide buffers and a proprietary variation of luminol designed to generate light from very small quantities of protein. This is called chemiluminescent detection (using chemical reactions to generate light) and the emitted photons are captured using the CCD camera of the BioChemi imaging unit. anti-mouse antibodies or anti-rabbit antibodies) is covalently linked to an enzyme (typically horseradish peroxidase, HRP) that catalyzes a localized light-generating reaction in the presence of substrate. This secondary antibody, which is raised against antibodies of a specific species, (e.g. The excess primary antibody is removed after a brief incubation period, then the blot is “washed” with buffer to remove non-specifically or weakly bound antibodies.Īfter this, a secondary antibody that recognizes the first (primary) antibody is incubated on the blot. For our purposes this target protein is GFP, the common “C-terminal tag” of all the fusion proteins. After preliminary incubation of the membrane in excess ‘inert’ protein (eg, milk) to block non-specific protein binding reactions, the membrane is then incubated with a primary antibody, which is an antibody that was generated to specifically recognize the ‘protein of interest’. To perform an immunoblot, the separated proteins within the gel are first transferred onto a membrane (usually nitrocellulose or PVDF) that can be treated with antibody solutions. Overview of a typical immunoblot procedure:Ī specific, single protein on a gel can be visualized through immunoblot analysis with an antibody that recognizes the protein of interest. * The average molecular weight of an amino acid is 110 daltons For ex., 1500 nucleotides means 500 codons and therefore 500 amino acids 500 x 110 = 55,000 daltons (or 55 kilodaltons, kDa). Divide this number by three to obtain the number of codons (which is the number of amino acids coded for) multiply this by 110* to obtain the approximate MW (in daltons) of your fusion protein. Begin “word count” with the first grey-shaded nucleotide, and count the entire area through and including the red “stop” TAA codon at the end of the ‘green’ GFP region. This value can be determined by counting the nucleotides in the ‘grey’ plus ‘green’ regions of your color-coded gene diagram. In today’s procedure, you will probe your blots with antibody to the GFP ‘tag’ on your fusion proteins this will ultimately reveal a protein band whose molecular weight (MW) is a combination of the GFP protein’s MW (~26 kDa) plus that of the protein product of your gene. 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# Floating point HDR color managed workflow For complex motion or parenting you can stack as many "transform" nodes as you want without quality loss thanks to transform concatenation. Clone nodes can also help process many nodes with complex behaviors. You can link parameters together and add expressions to automate their behavior. Shadertoy node brings the huge library of parametric animated textures in Natron. Multiple Texts can be generated on multiple layers to allow animating them freely. Vector shapes can also be drawn with the Roto node that allows you to draw many shapes and animate them as vectors. You can split your layers for separate animations of each part. Import vector file formats in Natron for logos. Path display and transform gizmo in the viewer gives you intuitive feedback. With powerful animation tools Natron lets you composite elements and then apply hundreds of built in effects, or add animated textures:Ĭurve editor let you precisely animate every element in Natron.The Dope Sheet let you keep track of your keyframes for easy retiming in a clean UI. > Need at least cameraprojection to be advetizable > updated cryptomatte must be included the community repository Lens distortion allows to integrate seamlessly with live tracked footage either with industry standard lens models or STMap Depth of field with depth pass (Community) relighting tools like "ReShade" and "SSAO" Screen Space Ambient Occlusion allows you to change lighting without going back to 3D rendering. Natron has the tools to composite your 3D renders easily Distortion based on a roto can be obtained with versatile "IDistort" node. Natron offers simple Bend/Swirl/Skew distortion of images or image based warps for creative heatwave/refraction effects. Lens flares come with many presets for quick yet fully customizable setup. With SeGrain you can precisely add grain to your images. Multiple denoising techniques are available. In Natron more than 100 image processing effects are at your disposal. HSVTool let's you select a color range for advanced color corrections. Tools for refining matte edges and color edges gives you professional results. Lightwrap get the right color integration. Additive keyer gets back the finest hairs. Can be setup to behave like Keylight (r) keyer. Natron provides many keyers to get the right matte in each situation. Roto shapes can be tracked for faster results. Each roto point has individual controls for feathering allowing compositors to capture plate motion blur in different areas of each roto-shape. Mask, feather, repeat! Natronʼs rotoscope node allows you to capture sub-pixel details. Luminance variation correction, manual keyframes help you get the right result. Natron includes Blenderʼs fast 2D tracker. Track difficult movements with ease and accuracy to get 1 point or perspective tracking. Natron features a node based interface, can import 3D renders from popular applications, and has hundreds of tools, effects and filters for creating high end visual effects! Nodes gives unlimited possibilities and clearer processing flow. > IMHO I think installing community scripts ans plugins should be strongly encouraged on the download page. With companion "community plugins" and "community scripts" it will get even more creative and efficient. Natron is a full featured production software. (Community) means community scripts or plugins functionality There are over ten multiplayer modes, some of which include characters and settings from previous Bond films. These missions are not available in the computer version of the game instead, the Windows version includes some additional bonus missions. There are five levels to your Aston Martin V12 Vanquish and other exotic but lethal sports cars. James Bond 007: Agent Under Fire Driven by a completely new and original storyline, James Bond 007: Agent Under Fire is a first-person action game tha. There are twelve missions, each combining stealth, combat, and Q’s trusty gadgets like your laser watch and X-ray eyeglasses.’ You have a variety of weapons at your disposal in your quest to stop the Phoenix Corporation, ranging from your trusty Walther to more advanced and prototype machinery. Throughout the game, you will be transported to over ten exotic locations, visiting all corners of the globe and confronting the stunning Bond women. In other words, thats what youd call, a Paris prelude. Because Drake’s plans are so well concealed, it is up to Bond, with the help of Q and his gadgets, to stop him at any cost. James Bond successfully saves the women and stops there second objective, to destroy the Eiffel Tower. Insert the James Bond 007: NightFire game disc. Does anyone happen to know how to make custom loadouts in multiplayer 2. FIOTOK Gamecube Controller, Classic Wired Controller for Wii Nintendo Gamecube (Blue & Red-2Pack) 4.5 out of 5 stars. If youre playing against a friend, plug another NINTENDO GAMECUBE. Still, later evidence has revealed that the corporation’s C.E.O., Raphael Drake, uses his company’s concept to find and use the warheads for his purposes. 007 NightFire Help Custom Multiplayer loadouts. The Phoenix International Corporation’s mission is to dismantle nuclear warheads worldwide. The story was good enough to convince players to get involved, though. Few players will remember the campaign/story missions, as they soon get washed up in the James Bond franchise that seems ecliptic. You take on the role of James Bond and are tasked with saving the world. In an era when the PS2 and original Xbox were breakneck technology, 007 Nightfire was quite the breakthrough.
Older PLCs can then talk to newer sensors, meters, scales, and other industrial monitoring and automation instruments.Ĭonfiguration can be done through a direct serial connection or via the network, so you can improve productivity and cut down on the amount of time required to troubleshoot a serial device on a factory floor or in a remote location. Translating older and newer protocols, the 1-Port Modbus Hardened Serial Server converts mismatched serial baud rates and settings, enabling the serial Modbus device to become a node on your Ethernet network. This rugged serial server gives you a simple way to migrate to an Ethernet-based industrial control system - without having to replace the older Modbus equipment you've already invested in. Includes a DIN mounting clip and spring.Įasily integrate an older serial Modbus device into your Ethernet Modbus network.Slim IP30 enclosure saves you on DIN rail space.Boasts high throughput with low latency.Equipped with a 10-/100-Mbps port for TCP connectivity.Features a DB9 port and terminal block for serial connections.Converts ASCII to RTU as well as ASCII or RTU to Modbus TCP.It can be easily integrated into most Modbus network configurations.Features a built-in Web server and easy-to-use management software.
Some places to start looking for support are Peertrainer, Weight Watchers and In Shape. Finding a good fit for each person is the key to keeping a person involved. Many communities have walking groups, nutritional and fitness groups or other peer support programs that can be helpful in maintaining a healthy lifestyle. Quitting smoking can very quickly reduce risk of developing metabolic syndrome and diabetes.ĭoctors, a NAMI Affiliate or community mental health program may offer suggestions of places to find peer support. These substances can change the way that the body digests and metabolizes food. Limiting alcohol, drug and nicotine use.Daily walking, running or another form of aerobic exercise can help you lose weight and keep your body healthy. A diet that is low in salt and fat and includes fruit and vegetables can help prevent obesity, high cholesterol, high blood pressure and diabetes. A doctor can check your weight, along with a measurement of your waist circumference and perform blood tests. Regular checkups with a primary care physician.A healthy lifestyle includes the following: Maintaining a healthy lifestyle is one of the best ways to prevent developing metabolic syndrome. This is a possible treatment option that can be discussed when seeing your psychiatrist or other health care provider.įortunately, there are ways to help prevent and detect metabolic syndrome. Some people who are taking SGAs may also find that medications used in the treatment of diabetes may be helpful in preventing metabolic syndrome. Selecting a medication should involve a careful review of the risks, as well as the benefits, the medicine offers. Preventing and Treating Metabolic Syndrome Guidelines for Preventing Healthcare Associated Pneumonia, 2003 Monitoring VAP CDC’s National Healthcare Safety Network Patient Safety Component includes surveillance methods to identify and track device-associated infections, such as central-line associated bloodstream infections. People of certain ethnicities, including African Americans, Latinos, Native Americans and Asian Americans.People with a family history of diabetes and metabolic syndrome.Other risk factors for metabolic syndrome include: This means that those with schizophrenia and bipolar disorder are at the highest risk for weight gain and metabolic syndrome because these medications are often used in treatment. The use of second-generation antipsychotics (SGAs), which can result in weight gain.Poor diet, lack of exercise, heavy smoking and abuse of substances can increase the risk of medical illnesses. Lifestyle choices, including diet, exercise and substance use.There are two connections between mental illness and metabolic syndrome: What Are the Connections Between Metabolic Syndrome and Mental Health Conditions? Due to the seriousness of this condition, early detection and intervention are critical. People with metabolic syndrome are at a high risk for developing many serious medical complications including type 2 diabetes, heart attacks and strokes. Metabolic syndrome is a medical condition that involves multiple related conditions including obesity, elevated blood sugar, high blood pressure and high cholesterol. Weight Gain Related to Psychiatric TreatmentsĮCT, TMS and Other Brain Stimulation Therapies What to Avoid with Psychiatric Medications Methylphenidate or Dexmethylphenidate (Concerta, Ritalin and others) Guidelines for Preventing Healthcare Associated Pneumonia, 2003ĬDC’s National Healthcare Safety Network Patient Safety Component includes surveillance methods to identify and track device-associated infections, such as central-line associated bloodstream infections.Dextromethorphan and Bupropion (Auvelity).
They’re not very strong right now, but they’ve got bravery in spades and plenty of fellow cats and dogs and a helpful little sprite to help them along the way. Meet the kings: a cat and dog reincarnated from kings long past and destined to save their kingdoms from the evil rulers who sit on their thrones in their stead, waging war like, well, cats and dogs. Cat Quest 2, though, despite its name, is a game built for cat people and dog people alike, because for the first time, you can be both. If you’re a dog person, you may have looked at Cat Quest and/or Cat Quest 2 and gone “No thanks, I’m a dog person.” And in Cat Quest 1, that would have been justified. You don’t mind the walks, the dog parks, and the copious amounts of time outside rain, snow, or shine, it just means you get some exercise and to meet other dogs. There’s just SO MUCH LOVE in every tail wag and bark and giddy hop. Your life revolves around them, and that’s okay, because they’re your best friend forever. You love them, and my god, do they love you back. You may not even mind when you do things like lay down on your keyboard it’s actually kinda cute. You know the joy when a cat decides that you’re “theirs” and you become their favorite chair. You enjoy the occasional interaction when they deign to acknowledge your existence, typically as pet-giver and know just how many times you can pet them before they’re done. You like being able to leave them alone with a huge bowl of food and water and clean litter and they’ll take care of themselves while you’re gone. If you’re a cat person, you like their independence. Yeah, there are people who love both (present reviewer included) but you know we still have our favorites. Most people break down into one of two categories: cat people or dog people. Cats seem to default to making enemies unless convinced otherwise. Dogs kind of default to making friends unless provoked. |